Polarized endosome dynamics engage cytoplasmic Par-3 that recruits dynein during asymmetric cell division
Xiang Zhao, Jason Q. Garcia, Kai Tong, Xingye Chen, Bin Yang, Qi Li, Zhipeng Dai, Xiaoyu Shi, Ian B. Seiple, Bo Huang, Su Guo
Abstract
daughter. This asymmetric segregation requires the activity of Par-3 and dynein motor complex. Using label retention expansion microscopy, we further detect Par-3 in the cytosol colocalizing the dynein light intermediate chain 1 (Dlic1) onto Dld endosomes. Par-3, Dlic1, and Dld are associated in protein complexes in vivo. Our data reveal an unanticipated mechanism by which cytoplasmic Par-3 directly polarizes Notch signaling components during ACD.
Topics & Concepts
EndosomeCell biologyCytoplasmCytosolOrganelleDyneinCell divisionCellBiologyChemistryMicrotubuleGeneticsBiochemistryIntracellularEnzymeWnt/β-catenin signaling in development and cancerDevelopmental Biology and Gene RegulationHippo pathway signaling and YAP/TAZ