Macrophage-Targeting and Hydrogen-Peroxide-Responsive Fluorescent Probe for Imaging of Inflammation In Vivo
Menglin Tao, Minghui Wang, Cuicui Jiang, Wenbin Liu, Wujuan Zhu, Xiang Shi, Zijuan Hai
Abstract
High Resolution Image Download MS PowerPoint Slide An uncontrolled immune response leads to many diseases; therefore, monitoring inflammation is crucial for the diagnosis of subsequent diseases, drug screening, and targeted therapy. Since the inflammatory response mainly occurs in macrophages, there is a need to develop more inflammatory probes with macrophage-targeting ability. Herein, we designed a macrophage-targeted and hydrogen-peroxide-activated fluorescent probe BOH-HCy-Man for real-time imaging of inflammation in vivo and a control probe BOH-HCy without the macrophage-targeting part. The larger rate constant toward H 2 O 2 led to the higher sensitivity of BOH-HCy-Man (19.1-fold) than BOH-HCy (10.2-fold) in vitro. With the help of its macrophage-targeting ability, BOH-HCy-Man possessed an additional 1.6-fold fluorescent enhancement in inflamed RAW 264.7 cells or 1.3-fold fluorescent enhancement in vivo than BOH-HCy . We expected that BOH-HCy-Man will be a powerful tool for early diagnosis of inflammation related diseases.