Photoelectrochemical bioanalysis of microRNA on yolk-in-shell Au@CdS based on the catalytic hairpin assembly-mediated CRISPR-Cas12a system
Ruijin Zeng, Jianhui Xu, Liling Lu, Qianyun Lin, Xue Huang, Lingting Huang, Mei‐Jin Li, Dianping Tang
Abstract
This work reports on the proof-of-concept of a photoelectrochemical (PEC) biosensor with a horseradish peroxidase-single stranded DNA-encoded magnetic bead (MB-ssDNA-HRP) signal probe cleaved by the catalytic hairpin assembly (CHA)-mediated clustered regularly interspaced short palindromic repeats (CRISPR)-Cas12a system for the quantification of microRNA (miR-21) by using yolk-in-shell Au@CdS as a photoactive material.
Topics & Concepts
CRISPRHorseradish peroxidaseBioanalysisChemistryAptamerBiosensorNanotechnologyDNACatalysisCombinatorial chemistryMaterials scienceEnzymeMolecular biologyBiochemistryGeneBiologyAdvanced biosensing and bioanalysis techniquesCRISPR and Genetic EngineeringBiosensors and Analytical Detection