Binding of His-tagged fluorophores to lipid bilayers of giant vesicles
Shreya Pramanik, Jan Steinkühler, Rumiana Dimova, Joachim P. Spatz, Reinhard Lipowsky
Abstract
Normalised fluorescence intensities of two His-tagged molecules, 6H-GFP and 6H-FITC, as functions of their molar concentrations. The solid curves depend on a single fit parameter, the equilibrium dissociation constants K d .
Topics & Concepts
FluorescenceVesicleChemistryDissociation constantMoleculeDissociation (chemistry)Lipid bilayerBiophysicsChemical physicsMembranePhysical chemistryOrganic chemistryBiochemistryBiologyPhysicsOpticsReceptorLipid Membrane Structure and BehaviorAdvanced Fluorescence Microscopy TechniquesSpectroscopy and Quantum Chemical Studies