Downregulation of miR‑7 and miR‑153 is involved in Helicobacter pylori CagA induced gastric carcinogenesis and progression
Yu Song, Dong Guo, Jiafei Liu, Lina Ge, Peng Liu, Ye-Min Qu, Haiyan Cong, Li Tian, Xin Chang, Yiran Wang, Liying Shao, Zong‐Jun Du, Ming‐Yi Wang
Abstract
<em>Helicobacter pylori</em> (<em>H. pylori</em>) infection plays a pivotal role in the development of gastric cancer (GC). However, the association between aberrant microRNAs (miRNAs/miRs) expression and <em>H. pylori</em>‑induced GC remains poorly understood. The present study reported that repeated infection of <em>H. pylori</em> caused the oncogenicity of GES‑1 cells in BALB/c Nude mice. miRNA sequencing revealed that both miR‑7 and miR‑153 were significantly decreased in the cytotoxin‑associated gene A (CagA) positive GC tissues and this was further confirmed in a chronic infection model of GES‑1/HP cells. Further biological function experiments and <em>in vivo</em> experiments validated that miR‑7 and miR‑153 can promote apoptosis and autophagy, inhibit proliferation and inflammatory response in GES‑1/HP cells. All the associations between miR‑7/miR‑153 and their potential targets were revealed via bioinformatics prediction and dual‑luciferase reporter assay. Particularly, downregulation of both miR‑7 and miR‑153 obtained an improved sensitivity and specificity in diagnosing <em>H. pylori</em> (CagA+)‑induced GC. The present study identified that the combination of miR‑7 and miR‑153 may be regarded as novel therapeutic targets in <em>H. pylori</em> CagA (+)‑associated GC.