Interstrand Aminoacyl Transfer in a tRNA Acceptor Stem-Overhang Mimic
Long-Fei Wu, Meng Su, Ziwei Liu, Samuel J. Bjork, John D. Sutherland
Abstract
-Acyl-aminoacyl residues are similarly transferred from a mixed anhydride with the 5'-phosphate to the 2',3'-diol but with a different dependence of efficiency and stereoselectivity on the 3'-overhang length and sequence. Given a prebiotically plausible and compatible synthesis of aminoacyl phosphate mixed anhydrides, these results suggest that RNA molecules with acceptor stem termini resembling modern tRNAs could have been spontaneously aminoacylated, in a stereoselective and chemoselective manner, at their 2',3'-diol termini prior to the onset of protein-catalyzed aminoacylation.
Topics & Concepts
AminoacylationChemistryTransfer RNAResidue (chemistry)Aminoacyl tRNA synthetaseAminoacyl-tRNAStereochemistryStereoselectivityKinetic resolutionEnantiomerRNABiochemistryCatalysisEnantioselective synthesisGeneRNA and protein synthesis mechanismsRNA modifications and cancerChemical Synthesis and Analysis