Brief guide to senescence assays using cultured mammalian cells
Eun Seok Kang, Chanhee Kang, Young‐Sam Lee, Seung‐Jae Lee
Abstract
Cellular senescence is a crucial biological process associated with organismal aging and many chronic diseases. Here, we present a brief guide to mammalian senescence assays, including the measurement of cell cycle arrest, change in cellular morphology, senescence-associated β-galactosidase (SA-β-gal) staining, and the expression of senescence-associated secretory phenotype (SASP). This work will be useful for biologists with minimum expertise in cellular senescence assays.
Topics & Concepts
SenescenceCellular senescenceCell biologyPhenotypeBiologyCellGeneticsGeneTelomeres, Telomerase, and SenescenceAdvanced biosensing and bioanalysis techniquesSingle-cell and spatial transcriptomics