Accurate Identification of Degraded Products of Aflatoxin B1 Under UV Irradiation Based on UPLC-Q-TOF-MS/MS and NMR Analysis
Yan-Duo Wang, Chenggang Song, Jian Yang, Tao Zhou, Yuyang Zhao, Jianchun Qin, Lanping Guo, Gang Ding
Abstract
Analysis, purification, and characterization of AFB 1 degraded products are vital steps for elucidation of the photocatalytic mechanism. In this report, the UPLC-Q-TOF-MS/MS technique was first coupled with purification and NMR spectral approaches to analyze and characterize degraded products of AFB 1 photocatalyzed under UV irradiation. A total of seventeen degraded products were characterized based on the UPLC-Q-TOF-MS/MS analysis, in which seven ones (1–7) including four (stereo) isomers (1,2, 5, and 6) were purified and elucidated by NMR experiments. According to the structural features of AFB 1 and degraded products (1–7), the possible photocatalytic mechanisms were suggested. Furthermore, AFB 1 and degraded products (1–7) were evaluated against different cell lines. The results indicated that the UPLC-Q-TOF-MS/MS technique combined with purification, NMR spectral experiments, and biological tests was an applicable integrated approach for analysis, characterization, and toxic evaluation of degraded products of AFB 1 , which could be used to evaluate other mycotoxin degradation processes.