A DNAzyme cascade for amplified detection of intracellular miRNA
Yanan Wu, Jing Li, Ke Quan, Xiangxian Meng, Xiaohai Yang, Jin Huang, Kemin Wang
Abstract
Inspired by the natural enzyme cascade reaction, an artificial DNAzyme cascade system is developed for the amplified detection of intracellular miR-141. The results showed that the method enormously enhanced the readout of the fluorescence signal and achieved a femtomolar detection limit.
Topics & Concepts
CascadeDeoxyribozymeIntracellularmicroRNAChemistryCascade reactionBiophysicsComputational biologyNanotechnologyMolecular biologyDNABiologyBiochemistryMaterials scienceChromatographyCatalysisGeneAdvanced biosensing and bioanalysis techniquesMicroRNA in disease regulationRNA Interference and Gene Delivery