Single-cell RNA sequencing reveals profibrotic roles of distinct epithelial and mesenchymal lineages in pulmonary fibrosis
Arun C. Habermann, Austin J. Gutierrez, Linh T. Bui, Stephanie L. Yahn, Nichelle I. Winters, Carla L. Calvi, Lance Peter, Mei-I Chung, Chase J. Taylor, Christopher S. Jetter, Latha Raju, Jamie Roberson, Guixiao Ding, Lori Wood, Jennifer M. S. Sucre, Bradley W. Richmond, Ana Serezani, Wyatt J. McDonnell, Simon B. Mallal, Matthew J. Bacchetta, James E. Loyd, Ciara M. Shaver, Lorraine B. Ware, Ross M. Bremner, Rajat Walia, Timothy S. Blackwell, Nicholas E. Banovich, Jonathan A. Kropski
Abstract
pathologic, ECM-producing epithelial cell population that was highly enriched in PF lungs. Multiple fibroblast subtypes were observed to contribute to ECM expansion in a spatially discrete manner. Together, these data provide high-resolution insights into the complexity and plasticity of the distal lung epithelium in human disease and indicate a diversity of epithelial and mesenchymal cells contribute to pathologic lung fibrosis.