Efficient and Stable Magnetic Chitosan-Lipase B from Candida Antarctica Bioconjugates in the Enzymatic Kinetic Resolution of Racemic Heteroarylethanols
Cristina Georgiana Spelmezan, László Csaba Bencze, Gabriel Katona, Florin Dan Irimie, Csaba Paizs, Monica Ioana Toșa
Abstract
Lipase B from Candida antarctica immobilized by covalent binding on sebacoyl-activated chitosan-coated magnetic nanoparticles proved to be an efficient biocatalyst (49.2–50% conversion in 3–16 h and >96% enantiomeric excess) for the enzymatic kinetic resolution of some racemic heteroarylethanols through transesterification with vinyl acetate. Under optimal conditions (vinyl acetate, n-hexane, 45 °C), the biocatalyst remains active after 10 cycles.
Topics & Concepts
Candida antarcticaKinetic resolutionLipaseChemistryChitosanEnzymeBiochemistryEnantioselective synthesisCatalysisEnzyme Catalysis and ImmobilizationCarbohydrate Chemistry and SynthesisClick Chemistry and Applications