The Duo of Osteogenic and Angiogenic Differentiation in ADSC-Derived Spheroids
А.А. Горкун, Daria Revokatova, И.М. Зурина, Д. А. Никишин, Polina Bikmulina, Peter Timashev, A. I Shpichka, Nastasia V. Kosheleva, T.D. Kolokoltsova, И. Н. Сабурина
Abstract
. However, simultaneous induction of multidirectional cell differentiation in spheroids from multipotent mesenchymal stromal cells (MMSCs) was not considered earlier. Here we show that arranging ADSCs into spheroids allows rapid and spontaneous acquiring of markers of both osteo- and angiogenesis compared with 2D culture. We further showed that this multidirectional differentiation persists in time, but is not influenced by classical protocols for osteo- or angio-differentiation. At the same time, ADSC-spheroids retain similar morphology and microarchitecture in different culture conditions. These findings can contribute to a better understanding of the fundamental aspects of autonomous regulation of differentiation processes and their cross-talks in artificially created self-organizing multicellular structures. This, in turn, can find a wide range of applications in the field of tissue engineering and regeneration.