Geometrical Characterization of Glass Nanopipettes with Sub-10 nm Pore Diameter by Transmission Electron Microscopy
Kazuki Shigyou, Linhao Sun, Riku Yajima, Shohei Takigaura, Masashi Tajima, Hirotoshi Furusho, Yousuke Kikuchi, Keisuke Miyazawa, Takeshi Fukuma, Azuma Taoka, Toshio Ando, Shinji Watanabe
Abstract
Glass nanopipettes are widely used for various applications in nanosciences. In most of the applications, it is important to characterize their geometrical parameters, such as the aperture size and the inner cone angle at the tip region. For nanopipettes with sub-10 nm aperture and thin wall thickness, transmission electron microscopy (TEM) must be most instrumental in their precise geometrical measurement. However, this measurement has remained a challenge because heat generated by electron beam irradiation would largely deform sub-10 nm nanopipettes. Here, we provide methods for preparing TEM specimens that do not cause deformation of such tiny nanopipettes.