Fenton reaction involvement on methyl orange biodegradation by brown-rot fungus Gloeophyllum trabeum
Adi Setyo Purnomo, Nur Elis Agustina Andyani, Refdinal Nawfa, Surya Rosa Putra
Abstract
The unwieldiness of Fenton reaction on biodegradation of methyl orange (MO) by brown-rot fungus Gloeophyllum trabeum was investigated based on Fe2+-dependent reaction. The degradation of MO (final concentration 75 mg/L) was performed in mineral salt media with and without Fe2+ with incubation period at 0, 7, 14, 21, and 28 days. Degradation analysis was performed using UV-Vis Spectrophotometer and LC-TOF/MS. The highest MO degradation occurred during 28 days incubation, which approximately 46.67% and 38.89% in medium with and without Fe2+, respectively, indicated that the presence of Fe2+ enhanced MO degradation. C15H18N3O5S− and C17H22N3O7S− were the metabolic products of MO degradation in mineral salt medium with Fe2+. The MO pathway by G. trabeum was stated by termination on double bond of the azo group and followed by hydroxylation to C15H18N3O5S−, and then undergoes methylation to C17H22N3O7S−. This study assumed that Fenton reaction might be involved in MO biodegradation by G. trabeum.