Phylogenetic analysis and in-depth characterization of functionally and structurally diverse CE5 cutinases
Vera Novy, Leonor Vieira Carneiro, Jae Ho Shin, Johan Larsbrink, Lisbeth Olsson
Abstract
. Docking analysis suggested that different clades of the phylogenetic tree may harbor enzymes with different modes of substrate interaction, involving a solvent-exposed catalytic triad, a lipase-like lid, or a clamshell-like active site possibly formed by flexible loops.
Topics & Concepts
CutinaseLipaseDocking (animal)Active siteCarbohydrate-binding modulePhylogenetic treeBiologyCatalytic triadEsteraseChemistryFungal proteinEnzymeStereochemistryBiochemistryYeastSaccharomyces cerevisiaeGlycoside hydrolaseGeneNursingMedicineMicroplastics and Plastic PollutionEnzyme Catalysis and ImmobilizationEnzyme-mediated dye degradation