Formulation of Kaempferol in Nanostructured Lipid Carriers (NLCs): A Delivery Platform to Sensitization of MDA-MB468 Breast Cancer Cells to Paclitaxel
Toofan Aghazadeh, Nuredin Bakhtiari, Isa Abdi, Fatemeh Ramezani, A Namdeo, S Brgeoddu, H Amawi, Jr Ashby, D Tukaramrao, P Trivedi, R Babu, A Tiwari, X Pang, X Zhang, Y Jiang, Q Su, Q Li, Z Li, K Lokhande, S Ballav, N Thosar, K Swamy, S Basu, M Ashrafizadeh, S Tavakol, Z Ahmadi, S Roomiani, R Mohammadinejad, S Samarghandian, S Ilk, N Saglam, M zgen, H Kahroba, B Ramezani, H Maadi, M Sadeghi, H Jaberie, F Ramezani, M More, S Pardeshi, C Pardeshi, G Sonawane, M Shinde, P Deshmukh, J Naik, A Kulkarni, S Raeisi, H Chavoshi, M Mohammadi, M Ghorbani, M Sabzichi, F Ramezani, F Khaki-Khatibi, M Zeinali, B Ramezani, M Sabzichi, J Mohammadian, H Hamishehkar, B Salehi, Cruz-Martins, M Butnariu, I Sarac, I.-C Bagiu, S Ezzat, J Wang, A Koay, H Sheridan, C Adetunji, V Rapalli, V Kaul, T Waghule, S Gorantla, S Sharma, A Roy, S Dubey, G Singhvi, E Landh, L Moir, L Reis, D Traini, P Young, H Ong, M Fathi, M Vincekovi, S Juri, M Viski, A Reek Jambrak, F Dons, S Mahmoudi, M Ghorbani, M Sabzichi, F Ramezani, H Hamishehkar, N Samadi, J Wang, M Kong, Z Zhou, D Yan, X Yu, X Cheng
Abstract
Drug delivery-based nanoparticle has been developing as a widespread innovation in cancer treatment protocols. Here, we investigated the role of Kaempferol (KAE) loaded in nanostructured lipid carriers (NLCs) to promote cytotoxicity, efficacy, and paclitaxel-dependent apoptosis in MDA-MB 468 breast cancer cells. Particle size distribution, scanning electron microscopy (SEM), zeta potential, and cellular uptake were harnessed to optimize and characterize of KAE -loaded NLCs. MTT assay was used to measure the cellular proliferation of cancer cells. The clarification of early and late apoptosis and their gene expression patterns was assessed by Annexin V/PI staining and real-time PCR, respectively. SEM images offered us a nasty particle size of 80 ± 3 nm to the Kaempferol formulated into NLCs. The IC50 values for KAE and paclitaxel determined 44 ± 0.52 μM and 1.75 ± 0.36 nM, respectively. The moderated cell proliferation from 56 ± 26.8% to 44 ± 3.9% (p < 0.05) was demonstrated by KAE loaded NLCs. Co-administration of KAE-loaded nanoparticles and paclitaxel into cancer cells significantly strengthens the percentage of apoptosis (p < 0.05). Our results recommend that KAE incorporated into NLCs as an anti-cancer adjuvant is a powerful technique that may be a useful delivery system to enhance chemotherapy agents' effect on breast cancer cells.