Effects of silica nanoparticles on morpho-histological and antioxidant activities of rice seedlings under drought stress

Abstract

Drought has a direct impact on rice growth performance at various stages. Nevertheless, the impact of nanoparticles on the rice seedlings under drought stress remained limited. Thus, a laboratory experiment was conducted to investigate the effects of different concentrations (300, 600, and 900 mg/L) of silica nanoparticles (SiNPs) on morpho-histological and biochemical features of two rice varieties (UMT-R and MR219) under drought stress (15 % PEG). The findings indicated a substantial reduction due to drought stress in the growth, protein, and antioxidant enzymes, while increasing the proline , malondialdehyde (MDA), and hydrogen peroxide (H 2 O 2 ) content, and negatively affecting the root histology. Results showed that SiNPs at 600 mg/L were managed to improve plant growth, root vigour, and enzymatic antioxidant levels. Under SiNPs treated plants, among UMT-R and MR219, the proline (1.947 and 1.748 in leaves, 2.010 and 1.890 U/mg protein in roots), peroxidase (POD) (2.850 and 3.512 in leaves, 2.521 and 2.952 U/mg protein in roots), superoxide dismutase (SOD) (3.958 and 4.558 in leaves, 4.296 and 4.606 U/mg protein in roots), and catalase (CAT) (2.171 and 2.289 in leaves, 1.897 and 2.050 U/mg protein in roots) were higher than the drought stress conditions. Meanwhile, the nonenzymatic antioxidant, MDA (0.974 and 0.812 in leaves, 0.842 and 0.778 U/mg Protein in roots), and H 2 O 2 (1.378 and 1.229 in leaves, 1.280 and 1.054 U/mg Protein in roots) showed an opposite trend. SiNPs application noticeably enhanced the histological features of roots under drought conditions. The current findings suggested that SiNPs at 600 mg/L can mitigate the adverse impacts of drought stress and enhance the plant's resistance. Further study should be carried out on the reproductive stage and post-harvest performance of drought-stress rice treated with SiNPs.

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