Synthesis of Trifluoromethylated Purine Ribonucleotides and Their Evaluation as <sup>19</sup>F NMR Probes
Mikołaj Chromiński, Marek R. Baranowski, Sebastian Chmielinski, Joanna Kowalska, Jacek Jemielity
Abstract
F NMR-based real-time enzymatic reaction monitoring is demonstrated with three different human nucleotide hydrolases (Fhit, DcpS, and cNIIIB). Substrate and product(s) resonances were sufficiently separated to enable effective tracking of each enzymatic activity of interest.
Topics & Concepts
PurineChemistryRadiochemistryOrganic chemistryEnzymeFluorine in Organic ChemistryCarbohydrate Chemistry and SynthesisRNA modifications and cancer