The Development of Microscopy for Super-Resolution: Confocal Microscopy, and Image Scanning Microscopy
Colin J. R. Sheppard
Abstract
Optical methods of super-resolution microscopy, such as confocal microscopy, structured illumination, nonlinear microscopy, and image scanning microscopy are reviewed. These methods avoid strong invasive interaction with a sample, allowing the observation of delicate biological samples. The meaning of resolution and the basic principles and different approaches to superresolution are discussed.
Topics & Concepts
MicroscopyScanning confocal electron microscopyConfocal microscopySuper-resolution microscopyResolution (logic)Optical sectioningMaterials scienceScanning ion-conductance microscopyLight sheet fluorescence microscopyOptical microscopeOpticsScanning probe microscopyNanotechnologyArtificial intelligenceComputer scienceScanning electron microscopePhysicsAdvanced Fluorescence Microscopy TechniquesNear-Field Optical MicroscopySpectroscopy Techniques in Biomedical and Chemical Research