SCASP: A Simple and Robust SDS-Aided Sample Preparation Method for Proteomic Research
Guohong Gan, Xiao Xu, Xi Chen, Xiufang Zhang, Jinling Wang, Chuan‐Qi Zhong
Abstract
SDS is widely used in sample preparation for proteomic research. However, SDS is incompatible with LC and electrospray ionization. SDS depletion is therefore required ahead of LC-MS analysis. Most of current SDS removal strategies are time consuming, laborious, and have low reproducibility. Here, we describe a method, SDS-cyclodextrin (CD)-assisted sample preparation, by which CD can bind to SDS and form CD-SDS complexes in solutions, allowing for direct tryptic digestion. We demonstrate that SDS-CD-assisted sample preparation is a simple, fast, and robust SDS-based sample preparation method for proteomics application.
Topics & Concepts
Sample preparationChromatographyChemistryProteomicsReproducibilityMass spectrometryElectrospray ionizationElectrosprayBiochemistryGeneAdvanced Proteomics Techniques and ApplicationsMass Spectrometry Techniques and ApplicationsMetabolomics and Mass Spectrometry Studies