Long‐Term <sup>15</sup>N Balance After Single‐Dose Input of <sup>15</sup>N‐Labeled NH<sub>4</sub><sup>+</sup> and NO<sub>3</sub><sup>−</sup> in a Subtropical Forest Under Reducing N Deposition
Danni Xie, Lei Duan, Gaoyue Si, Wenjing Liu, Ting Zhang, Jan Mulder
Abstract
Abstract Nitrogen (N) deposition in Europe and North America decreased in the 1990s, whereas N deposition in China began to decline in the early 2010s. The response of temperate forests to decreasing N deposition implied a delay recovery, but it remains unknown whether recovery in subtropical forests follows a similar trend. Therefore, the effects of decreased N deposition on N leaching were simulated in an N‐saturated forest in southwest China following ten years of N application 4.00 g N m −2 yr −1 . The addition of N (NH 4 + or NO 3 − ) was stopped in 2014. In 2017, a single in‐situ 15 N addition as NH 4 + or NO 3 − was performed to trace the fate of N under reducing N deposition. Combining the monitoring results of the N fluxes and 15 N fates, both the actual N leaching and contribution of a “new” N input to N leaching were significantly reduced in response to decreasing the N input. The termination of N addition resulted in immediate decreases in the N mineralization and immobilization rates, which were even lower than those in the control plots with moderate (naturally occurring) deposition reduction. The ratio of N leaching from mineralization was also reduced, implying the critical role of N mineralization in the recovery from N saturation. Because the forest was still N‐saturated, decreasing N deposition slightly reduced the N leaching to the gross N input ratio (including both N deposition and N mineralization). The significant decrease in the ratio and thus leaching recovery may occur due to a significant N deposition reduction.