Discovery of Glycosyltransferases Involved in the Biosynthesis of Ligupurpuroside B
Yihan Yang, Yanan Wu, Yibin Zhuang, Tao Liu
Abstract
In this study, we report the characterization of three glycosyltransferases involved in the biosynthesis of ligupurpuroside B, a complex acylated phenolic glycoside in Ligustrum robustum. UGT85AF8 catalyzed the formation of salidroside from tyrosol. UGT79G7, an osmanthuside A 1,3-rhamnosyltransferase, and UGT79A19, an osmanthuside B 1,4-rhamnosyltransferase, sequentially converted osmanthuside A into ligupurpuroside B. Orthologs of UGT79G7 were also discovered from other plants producing verbascoside. These rhamnosyltransferases expand the toolbox for the biosynthesis of natural products with various sugar chains.
Topics & Concepts
BiosynthesisGlycosyltransferaseVerbascosideChemistryTyrosolBiochemistryGlycosideGlycosylStereochemistryGenePhenolsMedicinal Plants and Bioactive CompoundsPhytochemistry and Biological ActivitiesPlant biochemistry and biosynthesis