Efficient and selective antibody modification with functionalised divinyltriazines
Andrew J. Counsell, Stephen J. Walsh, Naomi Robertson, Hannah F. Sore, David R. Spring
Abstract
A highly efficient disulfide rebridging strategy for the modification of monoclonal antibodies with substituted divinyltriazine linkers is reported. The reaction proceeds efficiently under mild conditions with near stoichiometric quantities of linker. This method of conjugation yields serum stable antibody conjugates with a controlled payload loading of 4.
Topics & Concepts
AntibodySurface modificationChemistryCombinatorial chemistryMedicineImmunologyPhysical chemistryMonoclonal and Polyclonal Antibodies ResearchHER2/EGFR in Cancer ResearchClick Chemistry and Applications