Targeting DAD1 gene with CRISPR-Cas9 system transmucosally delivered by fluorinated polylysine nanoparticles for bladder cancer intravesical gene therapy
Dongdong Tang, Yan Yang, Yangyang Li, Yuqing Li, Junqiang Tian, Li Yang, Hui Ding, Ghassan Bashir, Houhong Zhou, Qiuxia Ding, Ran Tao, Shaohua Zhang, Zhiping Wang, Song Wu
Abstract
The synthetized PLLF can transmucosally deliver the CRISPR-Cas9 system into orthotopic BCa tissues to improve intravesical instillation therapy for BCa. This work presents a new strategy for targeting DAD1 gene in the intravesical therapy for BCa with high potential for clinical applications.
Topics & Concepts
Genetic enhancementBladder cancerIn vivoCancer researchTransfectionPolylysineApoptosisMedicineChemistryCancerCell cultureBiologyGeneInternal medicineBiochemistryGeneticsBiotechnologyBladder and Urothelial Cancer TreatmentsCancer Research and TreatmentsTissue Engineering and Regenerative Medicine