PPRV-Induced Autophagy Facilitates Infectious Virus Transmission by the Exosomal Pathway
Yangli Wan, Yan Chen, Ting Wang, Bao Zhao, Wei Zeng, Leyan Zhang, Yanming Zhang, Shengyan Cao, Jingyu Wang, Qinghong Xue, Xuefeng Qi
Abstract
Autophagy plays an important role in PPRV pathogenesis. The role of exosomes in viral infections is beginning to be appreciated. The present study examined the role of autophagy in secretion of infectious PPRV from Vero cells. Our data provided the first direct evidence that ATG7-mediated autophagy enhances exosome secretion and exosome-mediated PPRV transmission. TSG101 may be involved in the sorting of the infectious PPRV RNA genomes into exosomes to facilitate the release of PPRV through the exosomal pathway. Inhibition of PPRV-induced autophagy or TSG101 expression could be used as a strategy to block exosome-mediated virus transmission.