Combined Analytical Approaches to Standardize and Characterize Biomaterials Formulations: Application to Chitosan-Gelatin Cross-Linked Hydrogels
Sofia Magli, Lorenzo Rossi, Cesare Consentino, Sabrina Bertini, Francesco Nicotra, Laura Russo
Abstract
A protocol based on the combination of different analytical methodologies is proposed to standardize the experimental conditions for reproducible formulations of hybrid hydrogels. The final hybrid material, based on the combination of gelatin and chitosan functionalized with methylfuran and cross-linked with 4-arm-PEG-maleimide, is able to mimic role, dynamism, and structural complexity of the extracellular matrix. Physical-chemical properties of starting polymers and finals constructs were characterized exploiting the combination of HP-SEC-TDA, UV, FT-IR, NMR, and TGA.
Topics & Concepts
Self-healing hydrogelsChitosanGelatinPolymerMaterials sciencePEG ratioMaleimideChemical engineeringExtracellular matrixChemistryNanotechnologyPolymer sciencePolymer chemistryComposite materialOrganic chemistryEconomicsEngineeringFinanceBiochemistryHydrogels: synthesis, properties, applications3D Printing in Biomedical ResearchCollagen: Extraction and Characterization