How to detect CRISPR with CRISPR – employing SHERLOCK for doping control purposes
Alina Paßreiter, Nana Naumann, Andreas Thomas, Nicolas Grogna, Philippe Delahaut, Mario Thevis
Abstract
adimistration study simulating a hypothetical gene doping scenario employing a mouse model revealed a detection window of 8 h after intravenous injection, supporting the principal applicability of the test strategy to authentic doping control samples in the future.
Topics & Concepts
CRISPRGenome editingComputational biologyGuide RNACas9Trans-activating crRNASubgenomic mRNANucleic acidDNABiologyComputer scienceGeneGeneticsCRISPR and Genetic EngineeringVirus-based gene therapy researchMosquito-borne diseases and control