Association Of Vitamin B12/Ferritin Deficiency In Cancer Patients with Methylomic Changes At Promotors of <i>TET</i> Methylcytosine Dioxygenases
Khaled A. Elawdan, Sabah Farouk, Salah Aref, Hamada Shoaib, Mohamed A El-Razik, Nasser H. Abbas, Mohamed Younis, Abeer A Alshambky, Hany Khalil
Abstract
Aim: To investigate potential DNA methylation in methylcytosine dioxygenases and correlation of TET genes with vitamin B12/ferritin levels in cancer patients. Materials & methods: 200 blood samples were obtained from both cancer patients and healthy individuals. Results: The expression of DNMT1, DNMT3a and DNMT3b was increased in patients with low vitamin B12 and ferritin levels, while the expression of MTR, TET1 and TET3 significantly decreased. DNA methylation analysis in patients with deficient vitamin B12/ferritin levels showed methylomic changes within the location 318/CG and 385/CG in the promoter region of TET1 and TET3, respectively. Conclusion: Vitamin B12/ferritin deficiency contributes to DNA methylation progress in cancer patients.