Single-cell RNA sequencing of human femoral head in vivo
Xiang Qiu, Ying Liu, Hui Shen, Zun Wang, Yun Gong, Junxiao Yang, Xiaohua Li, Huixi Zhang, Yu Chen, Cui Zhou, Wan‐Qiang Lv, Liang Cheng, Yihe Hu, Boyang Li, Wendi Shen, Xue-Zhen Zhu, Lijun Tan, Hong‐Mei Xiao, Hong‐Wen Deng
Abstract
) as novel bone metabolism-related genes. Additionally, we found that several subtypes of monocytes, T cells and B cells were related to bone metabolism. Cell-cell communication analysis showed that collagen, chemokine, transforming growth factor and their ligands have significant roles in the crosstalks between FHTCs. In particular, EPCs communicated with osteoblastic lineage cells closely via the "COL2A1-ITGB1" interaction pair. Collectively, this study provided an initial characterization of the cellular composition of the human FHTCs and the complex crosstalks between them at the single-cell level. It is a unique starting resource for in-depth insights into bone metabolism.