A validated simple LC-MS/MS method for quantifying trimethylamine<i>N</i>-oxide (TMAO) using a surrogate matrix and its clinical application
Yufei Li, Ji-Hyun Kang, Yujin Lee, Jae‐Yong Chung, Joo‐Youn Cho
Abstract
) higher than 0.996 in each validation batch. Moreover, both the intra-day and inter-day assays achieved satisfactory precision and accuracy results ranging from 1.65-7.15% and 96.36-111.43%, respectively. Further, this method was cross-validated using a human plasma matrix and applied to a clinical pharmacology study. Overall, these results demonstrate that the developed quantitation method is applicable in clinical research for monitoring disease progression and evaluating drug effects.
Topics & Concepts
Trimethylamine N-oxideChemistryChromatographyCalibration curveLiquid chromatography–mass spectrometryMatrix (chemical analysis)TrimethylamineMass spectrometryDetection limitBiochemistryMetabolomics and Mass Spectrometry StudiesPharmacogenetics and Drug MetabolismGut microbiota and health