Green synthesis of silver nanoparticles from extracts of <i>Pechuel-loeschea leubnitziae</i> : their anti-proliferative activity against the U87 cell line
Motshewa Justina Mofolo, Pauline Kadhila, Kazhila C. Chinsembu, Samson S. Mashele, Mamello Sekhoacha
Abstract
The application of green chemistry for the synthesis of nanoparticles is said to be environmentally friendly and cost efficient. The paper reports on the cell anti-proliferative activity of Pechuel-loeschea leubnitziae, synthesis of silver nanoparticles using P. leubnitziae and characterization. The UV–vis spectroscopy confirmed presence of a peak at 400 nm, and Fourier-transform infrared spectroscopy confirmed the presence of peaks from 700-4000 cm−1 correlating to AgNPs. Presence of silver, carbon and oxygen was confirmed by X-ray photoelectron spectroscopy and Energy-dispersive X-ray spectroscopy. Transmission electron microscopy showed a uniform spherical particle in the size of 100 nm, and scanning electron microscope showed spherical particles at a magnification of 100 µm. The extracts and the synthesized AgNPs were tested for cytotoxicity and anti-cancer activity against the Vero and U87 glioma cell lines. The results for both cell lines indicated that dichloromethane extract and AgNPs were cytotoxic and had potential anti-cancer properties.