Direct electron transfer-type bioelectrocatalysis by membrane-bound aldehyde dehydrogenase from Gluconobacter oxydans and cyanide effects on its bioelectrocatalytic properties
Taiki Adachi, Yuki Kitazumi, Osamu Shirai, Kenji Kano
Abstract
The bioelectrocatalytic properties of membrane-bound aldehyde dehydrogenase (AlDH) from Gluconobacter oxydans NBRC12528 were evaluated. AlDH exhibited direct electron transfer (DET)-type bioelectrocatalytic activity for acetaldehyde oxidation at several kinds of electrodes. The kinetic and thermodynamic parameters for bioelectrocatalytic acetaldehyde oxidation were estimated based on the partially random orientation model. Moreover, at the multi-walled carbon nanotube-modified electrode, the coordination of CN− to AlDH switched the direction of the DET-type bioelectrocatalysis to acetate reduction under acidic conditions. These phenomena were discussed from a thermodynamic viewpoint.
Topics & Concepts
Aldehyde dehydrogenaseAcetaldehydeChemistryElectron transferCyanideAldehydeHydrogenaseRedoxElectrodeInorganic chemistryEnzymeBiochemistryOrganic chemistryCatalysisPhysical chemistryEthanolMicrobial metabolism and enzyme functionMetalloenzymes and iron-sulfur proteinsMicrobial Fuel Cells and Bioremediation