Mechanism of hyperproteinemia-induced blood cell homeostasis imbalance in an animal model
Guang Wang, 苏州大学苏州医学院基础医学与生物科学学院, 江苏 苏州215123, 中国, Yongfeng Wang, Jianglan Li, Ruji Peng, Xinyin Liang, Xuedong Chen, Guihua Jiang, Jinfang Shi, Yang‐Hu Sima, Shiqing Xu, 苏州大学第一附属医院临床检验科, 江苏 苏州215006, 中国
Abstract
Hyperproteinemia is a metabolic disorder associated with increased plasma protein concentration (PPC) and is often clinically complicated by malignant diseases or severe infections. At present, however, research on the molecular mechanism underlying high PPC (HPPC) is scant. Here, an animal model of primary hyperproteinemia was constructed in an invertebrate (<i>Bombyx mori</i>) to investigate the effects of HPPC on circulating blood cells. Results showed that HPPC affected blood cell homeostasis, leading to increased reactive oxygen species levels, and induced programmed cell death dependent on the endoplasmic reticulum-calcium ion signaling pathway. HPPC induced the proliferation of blood cells, mainly granulocytes, by activating the Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling pathway. Supplementation with the endocrine hormone active substance 20E significantly reduced the impact of HPPC on blood cell homeostasis. Thus, we identified a novel signaling pathway by which HPPC affects blood cell homeostasis, which differs from hyperglycemia, hyperlipidemia, and hypercholesterolemia. In addition, we showed that down-regulation of gene expression of the hematopoietic factor <i>Gcm</i> could be used as a potential early detection indicator for hyperproteinemia.