The mutagenesis of a single site for enhancing or reversing the enantio- or regiopreference of cyclohexanone monooxygenases
Yujing Hu, Weihua Xu, Chenggong Hui, Jian Xu, Meilan Huang, Xianfu Lin, Qi Wu
Abstract
into larger tryptophan (W) enabled a significant enhancement of enantio- or regioselectivity toward structurally diverse substrates, moreover, a complete reversal of enantio- or regiopreference was realized by mutating F277 into a range of smaller amino acids (A/C/D/E/G/H/I/K/L/M/N/P/Q/R/S/T/V).
Topics & Concepts
ReversingCyclohexanoneChemistryMonooxygenaseMutagenesisSite-directed mutagenesisStereochemistryCombinatorial chemistryOrganic chemistryBiochemistryCatalysisEnzymeMutantMaterials scienceComposite materialGeneCytochrome P450Enzyme Catalysis and ImmobilizationPharmacogenetics and Drug MetabolismMicrobial Metabolic Engineering and Bioproduction