Intracellular delivery of therapeutic proteins through N-terminal site-specific modification
Si Si Liew, Chengwu Zhang, Jie Zhang, Hongyan Sun, Lin Li, Shao Q. Yao
Abstract
A versatile strategy for the intracellular delivery of functional proteins/antibodies was developed using N-terminal site-specific modification. Adopting orthogonal dual-labeling strategies, a cell-permeable RNase A prodrug was designed complementing N-terminal site-specific modification with lysine labeling. Upon successful cytosolic uptake, the prodrug showed reactive oxygen species (ROS)-dependent targeted cancer therapy.
Topics & Concepts
ProdrugIntracellularRNase PTerminal (telecommunication)ChemistryCancer therapyCellCytosolCell biologyBiochemistryCombinatorial chemistryComputational biologyCancerEnzymeBiologyComputer scienceRNAGeneticsGeneTelecommunicationsRNA Interference and Gene DeliveryMonoclonal and Polyclonal Antibodies ResearchPeptidase Inhibition and Analysis