An Improved Protocol for the Production of Lentiviral Vectors
Logan Y. Brown, Wendy Dong, Boris Kantor
Abstract
Lentiviral vectors are an ideal gene-delivery system for large gene-editing tools, such as the clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 system, due to their high packaging capacity and broad tropism. Here, we present a calcium phosphate-based protocol for lentiviral production and concentration for in vitro and in vivo use. This revised procedure has been optimized to ensure high viral titers and transduction efficiency and is scalable to meet specific production needs.
Topics & Concepts
CRISPRTransduction (biophysics)TropismViral vectorBiologyComputational biologyGenome editingGene deliveryGeneCas9Genetic enhancementComputer scienceVirologyGeneticsVirusRecombinant DNABiochemistryVirus-based gene therapy researchCRISPR and Genetic EngineeringViral Infectious Diseases and Gene Expression in Insects