Ethanol Leaf Extract of Hoslundia opposita in In Vivo Antioxidant and Hepatoprotective Activity Using an Animal Model
Fred Coolborn Akharaiyi, Odoligie Imarhiagbe, Lucky Efe Isunu, Adebayo Thomas Ajibola
Abstract
Introduction: The liver is a valuable organ responsible for detoxifying harmful substances from the body. It plays an essential role; hence the need to ensure its protection from damages. The management of liver diseases with orthodox medicine has been found to have side effects; consequently, there have been several calls on the use of alternative medicine for the effective management of liver diseases. Aim: . Materials and methods: antioxidants, and histopathology using an animal model. Results: ethanol leaf extract studied for 21 days suggested safety at a concentration of 400 mg/kgbw. Weight gain in the negative control was 16.91 g, while weight loss in the positive control mice was (13.96 g). 400 mg/kg was found as the LD50 of the plant extract. A decrease in uric acid, cholesterol, urea, creatinine, and bilirubin contents was observed in the single extract-treated mice and the paracetamol-induced but co-administered with extracts, while increased values were observed for protein and albumin contents. The positive control values of ALT, AST, and ALP were 66.74 ± 3.51 IU/L, 68.52 ± 3.63 IU/L, and 342 ± 3.04 IU/L, respectively, in the negative control, values were 48.16 ± 3.68 IU/L, 37.46 ± 1.52, and 89.34 ± 2.66 IU/L. There was a reduction in lipid peroxidation in the extract-treated and satellite groups. At the same time, increased values were observed for catalase and glutathione biochemical activities. The effects of a high dose of paracetamol were alleviated by the ethanol leaf extract over time. Conclusion: antioxidant values, and the hepatic damages caused by paracetamol toxicity were regulated on extract treatments, suggesting its use traditionally for the treatment of liver diseases.