SimPLIT: Simplified Sample Preparation for Large-Scale Isobaric Tagging Proteomics
Fernando J. Sialana, Theodoros I. Roumeliotis, Habib Bouguenina, Laura Chan Wah Hak, Hannah Wang, John Caldwell, Ian Collins, Rajesh Chopra, Jyoti S. Choudhary
Abstract
). Overall, SimPLIT is a robust method that can be easily implemented in any proteomics laboratory for medium-to-large scale TMT-based studies for deep profiling of cell lines.
Topics & Concepts
Isobaric processIsobaric labelingScale (ratio)ProteomicsSample preparationSample (material)ChromatographyQuantitative proteomicsComputer scienceChemistryPhysicsBiochemistryGeneThermodynamicsQuantum mechanicsUbiquitin and proteasome pathwaysProtein Degradation and InhibitorsAdvanced Proteomics Techniques and Applications