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Inhibiting NLRP3 inflammasome attenuates apoptosis in contrast-induced acute kidney injury through the upregulation of HIF1A and BNIP3-mediated mitophagy

Qisheng Lin, S. Li, Na Jiang, Haijiao Jin, Xinghua Shao, Xuying Zhu, Jingkui Wu, Minfang Zhang, Zhen Zhang, Jianxiao Shen, Wenyan Zhou, Leyi Gu, Renhua Lu, Zhaohui Ni

2020Autophagy390 citationsDOIOpen Access PDF

Abstract

BNIP3: BCL2/adenovirus E1B interacting protein 3; Ctrl: control; DAPI: 4',6-diamidino-2-phenylindole dihydrochloride; EGLN2/PHD1: egl-9 family hypoxia-inducible factor 2; HIF1A: hypoxia inducible factor 1, alpha subunit; H-E: hematoxylin and eosin; IL18: interleukin 18; IL1B: interleukin 1 beta; LAMP1: lysosomal-associated membrane protein 1; MAP1LC3B/LC3B: microtubule-associated protein 1 light chain 3 beta; mRNA: messenger RNA; NFKB/NF-κB: nuclear factor of kappa light polypeptide gene enhancer in B cells; NLRP3: NLR family, pyrin domain containing 3; NS: normal saline; PRKN/Parkin: parkin RBR E3 ubiquitin protein ligase; PINK1: PTEN induced putative kinase 1; RNA: ribonucleic acid; SEM: standard error of the mean; siRNA: small interfering RNA; TEM: transmission electron microscopy; TUBA/α-tubulin: tubulin, alpha; TUNEL: terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling; VDAC: voltage-dependent anion channel; WT: wild-type.

Topics & Concepts

MitophagyInflammasomeDownregulation and upregulationAutophagyPyroptosisCell biologyApoptosisAcute kidney injuryProgrammed cell deathMitochondrionCancer researchHIF1ABiologyMedicineInflammationImmunologyInternal medicineBiochemistryAngiogenesisGeneHeme Oxygenase-1 and Carbon MonoxideAutophagy in Disease and TherapyAcute Kidney Injury Research
Inhibiting NLRP3 inflammasome attenuates apoptosis in contrast-induced acute kidney injury through the upregulation of HIF1A and BNIP3-mediated mitophagy | Litcius