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Complete Genome Sequencing of Acinetobacter baumannii AC1633 and Acinetobacter nosocomialis AC1530 Unveils a Large Multidrug-Resistant Plasmid Encoding the NDM-1 and OXA-58 Carbapenemases

Ahmed Ghazi Alattraqchi, Farahiyah Mohd. Rani, Nor Iza A. Rahman, Salwani Ismail, David Cleary, Stuart C. Clarke, Chew Chieng Yeo

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Abstract

Bacteria of the genus Acinetobacter are important hospital-acquired pathogens, with carbapenem-resistant A. baumannii listed by the World Health Organization as the one of the top priority pathogens. Whole-genome sequencing of carbapenem-resistant A. baumannii AC1633 and A. nosocomialis AC1530, which were isolated from the main tertiary hospital in Terengganu, Malaysia, led to the discovery of a large, ca. 170-kb plasmid that harbored genes encoding the New Delhi metallo-β-lactamase-1 (NDM-1) and OXA-58 carbapenemases alongside genes that conferred resistance to aminoglycosides, macrolides, and sulfonamides.

Topics & Concepts

PlasmidAcinetobacter baumanniiBiologyMicrobiologyWhole genome sequencingMultiple drug resistanceAcinetobacterGenomeGeneticsGeneDrug resistanceBacteriaAntibioticsPseudomonas aeruginosaAntibiotic Resistance in BacteriaVibrio bacteria research studiesInfections and bacterial resistance