Stimulation of ammonia oxidizer and denitrifier abundances by nitrogen loading: Poor predictability for increased soil N<sub>2</sub>O emission
Yong Zhang, Feng Zhang, Diego Ábalos, Yiqi Luo, Dafeng Hui, Bruce A. Hungate, Pablo García‐Palacios, Yakov Kuzyakov, Jørgen E. Olesen, Uffe Jørgensen, Ji Chen
Abstract
Abstract Unprecedented nitrogen (N) inputs into terrestrial ecosystems have profoundly altered soil N cycling. Ammonia oxidizers and denitrifiers are the main producers of nitrous oxide (N 2 O), but it remains unclear how ammonia oxidizer and denitrifier abundances will respond to N loading and whether their responses can predict N‐induced changes in soil N 2 O emission. By synthesizing 101 field studies worldwide, we showed that N loading significantly increased ammonia oxidizer abundance by 107% and denitrifier abundance by 45%. The increases in both ammonia oxidizer and denitrifier abundances were primarily explained by N loading form, and more specifically, organic N loading had stronger effects on their abundances than mineral N loading. Nitrogen loading increased soil N 2 O emission by 261%, whereas there was no clear relationship between changes in soil N 2 O emission and shifts in ammonia oxidizer and denitrifier abundances. Our field‐based results challenge the laboratory‐based hypothesis that increased ammonia oxidizer and denitrifier abundances by N loading would directly cause higher soil N 2 O emission. Instead, key abiotic factors (mean annual precipitation, soil pH, soil C:N ratio, and ecosystem type) explained N‐induced changes in soil N 2 O emission. Altogether, these findings highlight the need for considering the roles of key abiotic factors in regulating soil N transformations under N loading to better understand the microbially mediated soil N 2 O emission.