Simultaneous Determination of 26 Pesticide Residues in Traditional Chinese Medicinal Leeches by Modified QuEChERS Coupled with HPLC–MS/MS
Ying Zhou, Yahui Yu, Qian Huang, Huixin Zheng, Ruyi Zhan, Luting Chen, Xingang Meng
Abstract
High Resolution Image Download MS PowerPoint Slide A Quick, Easy, Cheap, Effective, Rugged, and Safe (QuEChERS) pretreatment technique combined with HPLC–MS/MS was established to detect 26 pesticides in traditional Chinese medicinal leeches. The sample was extracted by acetonitrile solution with sodium acetate-0.1% (v/v) acetic acid as a buffer system, then cleaned up by a mixture of 750 mg of MgSO 4, 150 mg of C 18, and 150 mg of PSA, separated by an ACQUITY BEH C 18 column, and determined in the dynamic multiple reaction mode. Under the optimized conditions, the peak areas of the 26 pesticides in leeches showed good linearity ( r > 0.99) between their mass concentrations from 1 to 100 μg/L. At the spike levels of 10, 20, and 100 μg/kg, the recoveries of 26 pesticides in leeches were 72.9–101.6% with an RSD of 1.1–12.8%, an LOQ of 10 μg/kg, and an LOD of 0.1–5.4 μg/kg. This method is easy, rapid, sensitive, and practical and meets the requirements of pesticide residue detection standards.