Stimuli-responsive hydrogel microcapsules for the amplified detection of microRNAs
Wen-Hsin Chang, Yi‐Fang Lee, Yen-Wenn Liu, Itamar Willner, Wei‐Ching Liao
Abstract
pM and the limit of detection is 44.9 pM. Compared to direct microcapsular biosensing, the detection limit for miR-141 by microcapsules coupled with strand-displacement amplification is enhanced by four orders of magnitude.
Topics & Concepts
BiosensorDetection limitLoop-mediated isothermal amplificationLinear rangeAcrylamideMultiple displacement amplificationMaterials scienceDNAQuantum dotNanotechnologyRolling circle replicationChemistryPolymerChromatographyMonomerDNA replicationBiochemistryGenePolymerase chain reactionDNA extractionComposite materialAdvanced biosensing and bioanalysis techniquesRNA Interference and Gene DeliveryMicroRNA in disease regulation