Evaluation of antitumoral effect of Hibiscus sabdariffa extract on human breast cancer cells
Alessio Malacrida, Jacopo Erriquez, Maryamsadat Hashemi, Virginia Rodriguez‐Menendez, Arianna Cassetti, Guido Cavaletti, Mariarosaria Miloso
Abstract
-location from nucleus to perinuclear area and in cytoplasmic compartment. qRT-PCR and western blotting high-lighted that HsEF reduced ERα, BRCA1 and caveolin1 gene and protein expression in MCF-7 cells, but not in MDA-MB-231 cells. Moreover, we demonstrated that HsEF reduced proteasome activity, an increased autophagy, impair migration and invasion in both cell lines. ConclusionsOur data suggest HsEF has an antitumoral effects on both breast tumor cells examined and that ERα involvement could explain the differences observed between the two cell lines.
Topics & Concepts
Hibiscus sabdariffaViability assayTrypan blueMCF-7Cancer cellCell cultureMTT assayAutophagyCancer researchBlotChemistryCancerPharmacologyCellBiologyTraditional medicineApoptosisMedicineBiochemistryHuman breastInternal medicineGeneGeneticsHibiscus Plant Research StudiesPhytochemistry and biological activities of Ficus speciesAdvances in Cucurbitaceae Research