REVOLUTIONIZING PLANT BREEDING PROGRAMS WITH ADVANCEMENTS IN MOLECULAR MARKER-ASSISTED SELECTION
Asad Abbas, A ARSHAD, AU REHMAN, MS BUKHARI, Shah Zaman
Abstract
The molecular marker method has come a long way in the last 30 years. Genetic research has advanced from RFLPs to SNPs. Array technology was also used. Sequencing progress has also led to the creation of low-cost NGS systems with high throughput. Phenotyping has been very important in making this progress possible. Markers for important crops like rice, corn, and potatoes have been worked on, but markers for crops that aren't used much haven't been studied as much. Phylogenetics and molecular ecology are other areas that don't know much about molecular markers. Plant breeding and DNA modification have greatly changed since recent improvements to CRISPR technology. To the contrary, some MAS (marker-assisted selection) methods need to know about the genome beforehand, which makes the work even harder. Researchers in plant science might find the methods discussed in this review piece useful as a database. They could use them alone or with other sequence-level characters from different fields.