Generation and Characterization of Recombinant SARS-CoV-2 Expressing Reporter Genes
Kevin Chiem, Desarey Morales Vasquez, Jun‐Gyu Park, Roy N. Platt, Tim Anderson, Mark R. Walter, James J. Kobie, Chengjin Ye, Luis Martínez‐Sobrido
Abstract
Importantly, reporter-expressing rSARS-CoV-2 display similar growth kinetics and plaque phenotype that their wild-type counterpart (rSARS-CoV-2/WT), demonstrating their feasibility to identify drugs and/or neutralizing antibodies (NAbs) for the therapeutic treatment of SARS-CoV-2. Henceforth, these reporter-expressing rSARS-CoV-2 can be used to interrogate large libraries of compounds and/or monoclonal antibodies (MAb), in high-throughput screening settings, to identify those with therapeutic potential against SARS-CoV-2.
Topics & Concepts
BiologyRecombinant DNAVirologyReporter geneGeneGeneticsSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)Coronavirus disease 2019 (COVID-19)Molecular biologyComputational biologyGene expressionInfectious disease (medical specialty)MedicinePathologyDiseaseSARS-CoV-2 and COVID-19 ResearchViral gastroenteritis research and epidemiologyViral Infections and Outbreaks Research