Apta-sensor for selective determination of dopamine using chitosan-stabilized Prussian blue nanoparticles
Han Been Lee, Seong Eun Son, Gi Hun Seong
Abstract
into a hydroxyl radical (˙OH) and then oxidation of TMB by the ˙OH to produce a blue color. An aptamer-based colorimetric assay was made with the CS/PBNPs to detect DA at concentrations of 0.25-100 μM with a limit of detection (LOD) of 0.16 μM. For comparison, a gold nanoparticle (AuNP)-based apta-sensor detected DA in concentrations of 1-25 μM with a LOD of 0.55 μM. The recovery results of DA concentrations (0.25, 0.5, and 1 μM) from spiked human serum were 92.6%, 102.1%, and 103.9%, verifying the reliability and reproducibility of the CS/PBNP-based apta-sensor for determination of DA level in clinical applications. Moreover, compared to traditional immunoassay, this aptamer-based nanozyme activation/inhibition system needs no washing step, which is very useful to shorten the assay time and maintain high sensitivity.