Fluid shear stress enhances differentiation of jejunal human enteroids in Intestine-Chip
Jianyi Yin, Laxmi Sunuwar, Magdalena Kasendra, Huimin Yu, Chung‐Ming Tse, C. Conover Talbot, Tatiana Boronina, Robert N. Cole, Katia Karalis, Mark Donowitz
2020American Journal of Physiology-Gastrointestinal and Liver Physiology46 citationsDOIOpen Access PDF
Abstract
This study showed that polarized enteroid models in which there is no basolateral Wnt3a, are differentiated, regardless of the Wnt3a status of the apical media. The study supports the concept that in the human intestine villus differentiation is not an all or none phenomenon, demonstrating that at different days after lack of basolateral Wnt exposure, clusters of genes and proteins exist geographically along the villus with different domains having different functions.
Topics & Concepts
Shear stressCell biologyChemistryBiologyPhysicsMechanics3D Printing in Biomedical ResearchDigestive system and related healthCancer Cells and Metastasis