Exosome‐Coated <sup>10</sup>B Carbon Dots for Precise Boron Neutron Capture Therapy in a Mouse Model of Glioma In Situ
Juan Li, Jianglong Kong, Sihan Ma, Jiacheng Li, Meiru Mao, Kui Chen, Ziteng Chen, Jiaxin Zhang, Yanan Chang, Hui Yuan, Tong Liu, Zizhu Zhang, Gengmei Xing
Abstract
Abstract Novel boron‐containing carbon dots (BCDs) are designed and prepared for boron neutron capture therapy (BNCT) with superior water solubility and excellent optical property for tracking 10 B in vitro and in vivo. Encapsulation of BCDs using exosomes (Exos) from macrophages yields BCD–Exos of ≈100 nm. Fluorescent imaging shows that the BCD–Exos are internalized and distributed around the nuclei of U‐87‐MG glioma cells. BCD–Exos are also verified to cross the blood–brain barrier and significant accumulation in tumor tissue of the orthotopic U‐87‐MG glioma tumor‐bearing mice model 4 h after administration. Using inductively coupled plasma mass spectrometry (ICP‐MS), it is detected that 10 B in tumor tissue is 107.07 ± 1.58 ppm, and T/N ratios are enhanced from 2.03 ± 0.08 of boron phenylalanine (BPA) to 5.28 ± 0.29 of the BCD–Exos. In BNCT, the neutron radiation dose is 8.40 ± 0.12 Gy when a 500 mg kg −1 10 B dosage is given. Finally, there is a prominent BNCT effect of the BCD–Exos‐treated brain glioma in the mice model, and the survival ratio is 100% at the end of the experiment. The excellent curative effect of BNCT using BCD–Exos to brain glioma is achieved through adjusted biodistribution of boron in the cancer cell, enhanced T/N ratios, and the precise match between boron and neutron exposure in the site of the tumor in vivo with fluorescence imaging of BCD–Exos.