Developing a Simple Immunochromatography Assay for Clenbuterol with Sensitivity by One-Step Staining
Han Zhang, Lulu Wang, Xiaolin Yao, Zonghan Wang, Leina Dou, Lihong Su, Man Zhao, Jing Sun, Daohong Zhang, Jianlong Wang
Abstract
. Furthermore, this strategy was preliminarily verified by screening for CL in milk, pork tenderloin, and swine liver with recoveries ranging from 81 to 102%. Compared with conventional LFCIAs, the use of CBB as a signal label not only avoided the complicated material synthesis and surface modification process but also simplified the cross-linking with antibodies, meanwhile reducing the steric hindrance and increasing the possibility of immune recognition reactions, which was propitious for the effective utilization of antibodies. Taking advantages of the simplicity, rapidity, and cost-effectiveness, the CBB-based LFCIA may have potential for on-demand monitoring of general harmful small molecules by changing the kind of the staining antibody.